The test, which began as a crude physical inspection, has become more sophisticated over the years. In the 1970s and ’80s, the test was performed by a buccal smear — the scraping of cells from the inside of the mouth — and the sample studied for chromosomal material.
Over the past 40 years, dozens of female athletes tested in this manner have tested “positively” for maleness. That’s because these tests don’t measure “maleness” or “femaleness.” They measure — and not always reliably — the presence of a Y chromosome, or Y chromosomal material, which no small number of females have.